-Chain Gene Enhancer β Functional Analysis of the Murine TCR
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منابع مشابه
Identification of a Functional Nf-kb Binding Site in the Murine T Cell Receptor 02 Locus
The cr and /3 chains constituting the TCR are expressed specifically on T lymphoid cells after genomic rearrangements have led to the assembly of functional a and /3 chain genes (1) . T cell-specific transcriptional activation of the unrearranged gene segments is considered to be a prerequisite for the recombination process that apparently uses the same enzymatic machinery that rearranges the I...
متن کاملIdentification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
We have identified a sequence in the TCR beta 2 locus that is homologous to the kappa B site in the Ig kappa light chain enhancer. This element, TCR beta-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragment encompassing this site is preferentially active in T cells stimulated with phorbol esters or ...
متن کاملImmunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.
Multipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibi...
متن کاملIndistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus enhancers.
We have previously shown that the delta E3 site is an essential element for transcriptional activation by the human T-cell receptor (TCR) delta enhancer and identified two factors, NF-delta E3A and NF-delta E3C, that bound to overlapping core (TGTGGTTT) and E-box motifs within delta E3. In this study, we show that protein binding to the core motif is necessary but not sufficient for transcripti...
متن کاملGATA elements are necessary for the activity and tissue specificity of the T-cell receptor beta-chain transcriptional enhancer.
Three high-affinity binding sites for the GATA family of transcriptional regulators have been identified within the T-cell receptor beta-chain (TCR beta) transcriptional enhancer, and their functional significance has been determined in an effort to understand the T-cell specificity of the enhancer more fully. One site, TE4, is important for activity of the enhancer in T cells. Neither site TE1...
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تاریخ انتشار 2000